Boss L., Oehme R., Billig S., Birkemeyer C. & Layer G. (2017) The Radical SAM enzyme NirJ catalyzes the removal of two propionate side chains during heme d1 biosynthesis. FEBS J., in press. PUBMED: 29076625

Moore S.J., Sowa S.T., Schuchardt C., Deery E., Lawrence A.D., Ramos J.V., Billig S., Birkemeyer C., Chivers P.T., Howard M.J., Rigby S.E., Layer G. & Warren M.J. (2017) Elucidation of the biosynthesis of the methane catalyst coenzyme F430. Nature, 543, 78-82. PUBMED: 28225763

Layer G., Krausze J. & Moser J. (2017) Reduction of chemically stable multibonds: Nitrogenase-like biosynthesis of tetrapyrroles. Adv. Exp. Med. Biol., 925, 147-161. PUBMED: 27957709

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Layer G. (2016) Siroheme decarboxylase in complex with iron-uroporphyrin III. in "Encyclopedia of Inorganic and Bioinorganic Chemistry", ed R.A. Scott, John Wiley, Chichester. DOI: 10.1002/9781119951438.eibc2444.

Kühner M., Schweyen P., Hoffmann M., Vazquez Ramos J., Reijerse E.J., Lubitz W., Bröring M. & Layer G. (2016) The auxiliary [4Fe-4S] cluster of the Radical SAM heme synthase from Methanosarcina barkeri is involved in electron transfer. Chem. Sci., 7, 4633-43. DOI: 10.1039/C6SC01140C

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Adamczack J., Hoffmann M., Papke U., Haufschildt K., Nicke T., Bröring M., Sezer M., Weimar R., Kuhlmann U., Hildebrandt P. & Layer G. (2014) NirN from Pseudomonas aeruginosa is a novel electron-bifurcating dehydrogenase catalyzing the last step of heme d1 biosynthesis. J. Biol. Chem., 289, 30753-62. PUBMED: 25204657

Haufschildt K., Schmelz S., Kriegler T.M., Neumann A., Streif J., Arai H., Heinz D.W. & Layer G. (2014) The crystal structure of siroheme decarboxylase in complex with iron-uroporphyrin III reveals two essential histidine residues. J. Mol. Biol., 426, 3272-86. PUBMED: 25083922

Kühner M., Haufschildt K., Neumann A., Storbeck S., Streif J. & Layer G. (2014) The alternative route to heme in the methanogenic archaeon Methanosarcina barkeri. Archaea, 2014, Article ID 327637. PUBMED: 24669201

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Nicke T., Schnitzer T., Münch K., Adamczack J., Haufschildt K., Buchmeier S., Kucklick M., Riedel K., Felgenträger U., Jänsch L. & Layer G. (2013) Maturation of the cytochrome cd1 nitrite reductase NirS from Pseudomonas aeruginosa requires transient interactions between the three proteins NirS, NirN and NirF. Biosci. Rep., 33, art:e00048. PUBMED: 23683062

Kaufholz A.-L., Hunter G. A., Ferreira G. A., Lendrihas T., Hering V., Layer G., Jahn M. & Jahn D. (2013) Aminolevulinic acid synthase of Rhodobacter capsulatus - high resolution kinetic investigation of the structural basis for substrate binding and catalysis. Biochem. J., 451, 205-16. PUBMED: 23363548

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Abicht H.K., Martinez J., Layer G., Jahn D. & Solioz M. (2012) Lactococcus lactis HemW (HemN) is a heme-binding protein with a putative role in heme trafficking. Biochem. J., 442, 335-43. PUBMED: 22142238

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Layer G. & Warren M.J. (2011) Biosynthesis of siroheme, cofactor F430 and heme d1. Handbook of Porphyrin Science19, 111-38.

Storbeck S., Saha S., Krausze J., Klink B.U., Heinz D.W. & Layer G. (2011) Crystal structure of the heme d1 biosynthesis enzyme NirE in complex with its substrate reveals new insights into the catalytic mechanism of S-adenosyl-L-methionine dependent uroporphyrinogen III methyltransferases. J. Biol. Chem., 286, 26754-67. PUBMED: 21632530

Layer G., Jahn D. & Jahn M. (2011) Heme biosynthesis. Handbook of Porphyrin Science15, 159-215.

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Storbeck S., Rolfes S., Raux-Deery E., Warren M.J., Jahn D. & Layer G. (2010) A novel pathway for the biosynthesis of heme in Archaea: Genome-based bioinformatic predictions and experimental evidence. Archaea, 2010, ID 175050. PUBMED: 21197080

Wollers S., Layer G., Garcia-Serres R., Signor L., Clemancey M., Latour JM., Fontecave M. & Ollagnier-de Choudens S. (2010) Iron-sulfur (Fe-S) cluster assembly: the SufBCD complex is a new type of Fe-S scaffold with a flavin redox cofactor. J. Biol. Chem., 285, 23331-41. PUBMED: 20460376

Layer G., Reichelt J., Jahn D. & Heinz D.W. (2010) Structure and function of enzymes in heme biosynthesis. Protein Science, 19, 1137-61. PUBMED: 20506125

Rand K., Noll C., Schiebel H.M., Kemken D., Dülcks T., Kalesse M., Heinz D.W. & Layer G. (2010)  Oxygen-independent coproporphyrinogen III oxidase HemN utilizes harderoporphyrinogen as a reaction intermediate during conversion of coproporphyrinogen III into protoporphyrinogen IX. Biol. Chem., 391, 55-63. PUBMED: 19919179

Layer G., Jahn D., Deery E., Lawrence A.D. & Warren M.J. (2010) Biosynthesis of heme and vitamin B12. in "Comprehensive natural products II Chemistry and Biology" (Mander, L., Lui, H.-W.) Elsevier: Oxford; 7, 445–99.

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Storbeck S., Walther J., Müller J., Parmar V., Schiebel H.M., Kemken D., Dülcks T., Warren M.J. & Layer G. (2009) The Pseudomonas aeruginosa nirE gene encodes the S-adenosyl-L-methionine dependent uroporphyrinogen III methyltransferase required for heme d1 biosynthesis. FEBS J., 276, 5973-82. PUBMED: 19754882

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vor 2008

Layer G., Gaddam S.A., Ayala-Castro C.N., Ollagnier-de Choudens S., Lascoux D., Fontecave M. & Outten F.W. (2007) SufE transfers sulfur from SufS to SufB for iron-sulfur cluster assembly. J. Biol. Chem., 282, 13342-50. PUBMED: 17350958

Layer G., Ollagnier-de Choudens S., Sanakis Y. & Fontecave M. (2006) Iron-sulfur cluster biosynthesis: characterization of Escherichia coli CyaY as an iron donor for the assembly of [2Fe-2S] clusters in the scaffold IscU. J. Biol. Chem., 281, 16256-63. PUBMED: 16603772

Layer G., Pierik A.J., Trost M., Rigby S.E., Leech H.K., Grage K., Breckau D., Astner I., Jänsch L., Heathcote P., Warren M.J., Heinz D.W. & Jahn D. (2006) The substrate radical of Escherichia coli oxygen-independent coproporphyrinogen III oxidase HemN. J. Biol. Chem., 281, 15727-34. PUBMED: 16606627

Reents H., Gruner I., Harmening U., Böttger L.H., Layer G., Heathcote P., Trautwein A.X., Jahn D. & Härtig E. (2006) Bacillus subtilis Fnr senses oxygen via a [4Fe-4S] cluster coordinated by three cysteine residues without change in the oligomeric state. Mol. Microbiol., 60, 1432-45. PUBMED: 16796679

Layer G., Grage K., Teschner T., Schünemann V., Breckau D., Masoumi A., Jahn M., Heathcote P., Trautwein A.X. & Jahn D. (2005) Radical S-adenosylmethionine enzyme coproporphyrinogen III oxidase HemN: functional features of the [4Fe-4S] cluster and the two bound S-adenosyl-L-methionines. J. Biol. Chem., 280, 29038-46. PUBMED: 15967800

Layer G., Kervio E., Morlock G., Heinz D.W., Jahn D., Retey J. & Schubert W.D. (2005) Structural and functional comparison of HemN to other radical SAM enzymes. Biol. Chem., 386, 971-80. PUBMED: 16218869

Layer G., Heinz D.W., Jahn D. & Schubert W.D. (2004) Structure and function of Radical SAM enzymes. Curr. Opin. Chem. Biol., 8, 468-76. PUBMED: 15450488

Layer G., Moser J., Heinz D.W., Jahn D. & Schubert W.D. (2003) Crystal structure of coproporphyrinogen III oxidase reveals cofactor geometry of Radical SAM enzymes. EMBO J., 22, 6214-24. PUBMED: 14633981

Breckau D., Mahlitz E., Sauerwald A., Layer G. & Jahn D. (2003) Oxygen-dependent coproporphyrinogen III oxidase (HemF) from Escherichia coli is stimulated by manganese. J. Biol. Chem., 278, 46625-31. PUBMED: 12975365

Baysse C., Matthijs S., Schobert M., Layer G., Jahn D. & Cornelis P. (2003) Co-ordination of iron acquisition, iron porphyrin chelation and iron-protoporphyrin export via the cytochrome c biogenesis protein CcmC in Pseudomonas fluorescens. Microbiology, 149, 3543-52. PUBMED: 14663086

Layer G., Verfürth K., Mahlitz E. & Jahn D. (2002) Oxygen-independent coproporphyrinogen-III oxidase HemN from Escherichia coli. J. Biol. Chem., 277, 34136-42. PUBMED: 12114526

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letzte Änderung: 06.11.2017


Prof. Dr. Gunhild Layer
Institut für Biochemie
Universität Leipzig
Brüderstraße 34
D-04103 Leipzig

Telefon: +49 341 97-36996
Telefax: +49 341 97-36919

Petra Hartung

Telefon: +49 341 97-36910
Telefax: +49 341 97-36919