Sonnendecker C, Wei R, Kurze E, Wang J, Oeser T, Zimmermann W. 2017. Efficient extracellular recombinant production and purification of a Bacillus cyclodextrin glucanotransferase in Escherichia coli. Microbial Cell Factories 16:87

Wei R, Zimmermann W. 2017. Biocatalysis as a green route for recycling the recalcitrant plastic polyethylene terephthalate. Microbial Biotechnology

Wei R, Zimmermann W. 2017. Microbial enzymes for the recycling of recalcitrant petroleum-based plastics: how far are we? Microbial Biotechnology

Schmidt J, Wei R, Oeser T, Dedavid e Silva LA, Breite D, Schulze A, Zimmermann W. 2017. Degradation of polyester polyurethane by bacterial polyester hydrolases. Polymers 9(2): 65

Roth C, Weizenmann N, Bexten N, Saenger W, Zimmermann W, Maier T, Sträter N. 2017. Amylose recognition and ring-size determination of amylomaltase. Science Advances 3(1): e1601386

Tan GYT, Zimmermann W, Lee K-H, Lan JC-W, Yim HS, Ling TC, Ng HS. 2017. Recovery of mangostins from Garcinia mangostana peels with an aqueous micellar biphasic system. Food and Bioproducts Processing 102:233-240

Rakchai N, H-Kittikun A, Zimmermann W. 2017. The production of immobilized whole-cell lipase from Aspergillus nomius ST57 and the enhancement of the synthesis of fatty acid methyl esters using a two-step reaction. Journal of Molecular Catalysis B: Enzymatic


Assaf KI, Gabela D, Zimmermann W, Nau WM. 2016. High-affinity host–guest chemistry of large-ring cyclodextrins. Organic and Biomolecular Chemistry 14:7702-7706

Schmidt J, Wei R, Oeser T, Belisário-Ferrari MR, Barth M, Then J, Zimmermann W. 2016. Effect of Tris, MOPS and phosphate buffers on the hydrolysis of polyethylene terephthalate films by polyester hydrolases. FEBS Open Bio 6(9):919-927

Barth M, Honak A, Oeser T, Wei R, Belisário-Ferrari MR, Then J, Schmidt J, Zimmermann W. 2016. A dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films. Biotechnology Journal 11(8):1082-1087

Then J, Wei R, Oeser T, Gerdts A, Schmidt J, Barth M, Zimmermann W. 2016. A disulfide bridge in the calcium binding site of a polyester hydrolase increases its thermal stability and activity against polyethylene terephthalate. FEBS Open Bio 6(5):425-432

Wei R, Oeser T, Schmidt J, Meier R, Barth M, Then J, Zimmermann W. 2016. Engineered bacterial polyester hydrolases efficiently degrade polyethylene terephthalate due to relieved product inhibition. Biotechnology and Bioengineering 113(8):1658-1665


Barth M, Wei R, Oeser T, Then J, Schmidt J, Wohlgemuth F, Zimmermann W. 2015. Enzymatic Hydrolysis of polyethylene terephthalate films in an ultrafiltration membrane reactor. Journal of Membrane Science 194:182–187

Melzer S, Sonnendecker C, Föllner C, Zimmermann W. 2015. Step wise error-prone PCR and DNA shuffling changed the pH activity range and product specifity of the cyclodextrin glucanotransferase from an alkaliphilic Bacillus sp. FEBS Open Bio 5(1):528–534

Barth M, Oeser T, Wei R, Then J, Schmidt J, Zimmermann W. 2015. Effect of hydrolysis products on the enzymatic degradation of polyethylene terephthalate nanoparticles by a polyester hydrolase from Thermobifida fusca. Biochemical Engineering Journal 93:222–228

Ottenheim C, Werner KA, Zimmermann W, Wu JC. 2015. Improved endoxylanase production and colony morphology of Aspergillus niger DSM 26641 by γ-ray induced mutagenesis. Biochemical Engineering Journal 94:9–14

Wang J, Wei R, Tian J, Yang N, Xu X, Zimmermann W, Jin Z. 2015. Multi-wavelength colorimetric determination of large-ring cyclodextrin content for the cyclization activity of 4-α-glucanotransferase. Carbohydrate Polymers 122:329–335

Then J, Wei R, Oeser T, Barth M, Belisario-Ferrari MR, Schmidt J, Zimmermann W. 2015. Ca2+ and Mg2+ binding site engineering increases the degradation of polyethylene terephthalate films by polyester hydrolases from Thermobifida fusca. Biotechnology Journal 10:592–598

Ottenheim C, Meyer K, Zimmermann W, Wu JC. 2015. Isolation of filamentous fungi exhibiting high endoxylanase activity in lignocellulose hydrolysate. Applied Biochemistry and Biotechnology 175:2066–2074

Berezina N, Yada B, Godfroid T, Senechal T, Wei R, Zimmermann W. 2015. Enzymatic surface treatment of poly (3-hydroxybutyrate) (PHB), and poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). Journal of Chemical Technology & Biotechnology 90:2036–2039


Watanasatitarpa S, Rudeekulthamrong P, Krusong K, Srisimarat W, Zimmermann W, Pongsawasdi P, Kaulpiboon J. 2014. Molecular mutagenesis at Tyr-101 of the amylomaltase transcribed from a gene isolated from soil DNA. Applied Biochemistry and Microbiology 50:243–252

Roth C, Wei R, Oeser T, Then J, Föllner C, Zimmermann W, Sträter N. 2014. Structural and functional studies on a thermostable polyethylene terephthalate degrading hydrolase from Thermobifida fusca. Applied Microbiology and Biotechnology 98:7815–7823

Sawasdee K, Rudeekulthamrong P, Zimmermann W, Murakami S, Pongsawasdi P, Kaulpiboon J. 2014. Direct cloning of gene encoding a novel amylomaltase from soil bacterial DNA for large-ring cyclodextrin production. Applied Biochemistry and Microbiology 50:17–24

Wei R, Oeser T, Barth M, Weigl N, Luebs A, Schulz-Siegmund M, Hacker MC, Zimmermann W. 2014. Turbidimetric analysis of the enzymatic hydrolysis of polyethylene terephthalate nanoparticles. Journal of Molecular Catalysis B: Enzymatic 103:72–78

Wei R, Oeser T, Then J, Kühn N, Barth M, Schmidt J, Zimmermann W. 2014. Functional characterization and structural modeling of synthetic polyester-degrading hydrolases from Thermomonospora curvata. AMB Express 4:44

Wei R, Oeser T, Zimmermann W. 2014. Synthetic polyester-hydrolyzing enzymes from thermophilic actinomycetes. Advances in Applied Microbiology 89:267–305

Ottenheim C, Verdejo C, Zimmermann W, Wu JC. 2014. Hemicellulase production by Aspergillus niger DSM 26641 in hydrothermal palm oil empty fruit bunch hydrolysate and transcriptome analysis. Journal of Bioscience and Bioengineering 118:696–701


Ciardelli G, Guebitz G, Zimmermann W. 2013. Editorial, Reactive and Functional Polymers 73:1327


Srisimarat W, Kaulpiboon J, Krusong K, Zimmermann W, Pongsawasdi P. 2012. Altered large-ring cyclodextrin product profile due to a mutation at Tyr-172 in the amylomaltase of Corynebacterium glutamicum. Applied and Environmental Microbiology 78:7223–7228

Wei R, Oeser T, Billig S, Zimmermann W. 2012. A high-throughput assay for enzymatic polyester hydrolysis activity by fluorimetric detection. Biotechnology Journal 7:1517–1521

Chaiyaso T, Seesuriyachan P, Zimmermann W, H-Kittikun A. 2012. Purification and characterization of  lipase from newly isolated Burkholderia multivorans PSU-AH130 and its application for biodiesel production. Annals of Microbiology 62:1615–1624

H-Kittikun A, Prasertsan P, Zimmermann W, Seesuriyachan P, Chaiyaso T. 2012. Sugar ester synthesis by thermostable lipase from Streptomyces thermocarboxydus ME168. Applied Biochemistry and Biotechnology 166:1969–1982


Zimmermann W, Billig S. 2011. Enzymes for the biofunctionalization of poly(ethylene terephthalate). Advances in Biochemical Engineering/Biotechnology 125:97–120

Herrero Acero E, Ribitsch D, Steinkellner G, Gruber K, Greimel K, Eiteljoerg I, Trotscha E, Wei R, Zimmermann W, Zinn M, Cavaco-Paulo A, Freddi G, Schwab H, Guebitz G. 2011. Enzymatic surface hydrolysis of PET: effect of structural diversity on kinetic properties of cutinases from Thermobifida. Macromolecules 44:4632–4640

Ellouze F, Amar NB, Mokhtar MN, Zimmermann W, Deratani A. 2011. Fractionation of homologous CD6 to CD60 cyclodextrin mixture by ultrafiltration and nanofiltration. Journal of Membrane Science 374:129–137

Yenpetch W, Packdibamrung K, Zimmermann W, Pongsawasdi P. 2011. Biochemical properties and cyclodextrin production profiles of isoforms of cyclodextrin glycosyltransferase. Journal of Inclusion Phenomena and Macrocyclic Chemistry 70:377–383

Srisimarat W, Powviriyakul A, Kaulpiboon J, Krusong K, Zimmermann W, Pongsawasdi P. 2011. A novel amylomaltase from Corynebacterium glutamicum and analysis of the large-ring cyclodextrin products. Journal of Inclusion Phenomena and Macrocyclic Chemistry 70:369–375

Yenpetch W, Packdibamrung K, Zimmermann W, Pongsawasdi P. 2011. Evidence of the involvement of asparigine deamidation in the formation of cyclodextrin glycosyltransferase isoforms in Paenibacillus sp. RB01. Molecular Biotechnology 47:234–242


Billig S, Oeser T, Birkemeyer C, Zimmermann W. 2010. Hydrolysis of cyclic poly(ethylene terephthalate) trimers by a carboxylesterase from Thermobifida fusca KW3. Applied Microbiology and Biotechnology 87:1753–1764

Dittmann M, Sauermann J, Seidel R, Zimmermann W, Engelhard M. 2010. Native chemical ligation of hydrophobic peptides in organic solvents. Journal of Peptide Science 16:558–562

Oeser T, Wei R, Baumgarten T, Billig S, Föllner C, Zimmermann W. 2010. High level expression of a hydrophobic poly(ethylene terephthalate)-hydrolyzing carboxylesterase from Thermobifida fusca KW3 in Escherichia coli BL21(DE3). Journal of Biotechnology 146:100–104

Chen S, Wu J, Su L, Billig S, Zimmermann W, Chen J. 2010. Biochemical characterization of the cutinases from Thermobifida fusca. Journal of Molecular Catalysis B: Enzymatic 63:121–127

Kaulpiboon J, Pongsawasdi P, Zimmermann W. 2010. Altered product specificity of a cyclodextrin glycosyltransferase by molecular imprinting with cyclomaltododecaose. Journal of Molecular Recognition 23: 480–485

Korpecka J, Heumann S, Billig S, Zimmermann W, Zinn M, Ihssen J, Cavaco-Paulo A, Guebitz G. 2010. Hydrolysis of cutin by PET-hydrolases. Macromolecular Symposia 296:342–346


Feuerhack A, Alisch-Mark M, Kisner A, Pezzin S, Zimmermann W, Andreaus J. 2008. Biocatalytic surface modification of knitted fabrics made of poly(ethylene terephthalate) with hydrolytic enzymes from Thermobifida fusca KW3b. Biocatalysis and Biotransformation, 26:357-364.


Endo T, Ogawa, N, Nagase H, Sambo H, Takaha T, Terada Y, Zimmermann W, Ueda H. 2007. Production of large-ring cyclodextrins composed of 9-21 a-D-glucanopyranose units by cyclodextrin glucanotransferase – effects of incubation temperature and molecular weight of amylose. Heterocycles 74:991-997.

Nimchua T, Punnapayak H, Zimmermann W. 2007. Comparison of the hydrolysis of polyethylene terephthalate (PET) fibers by a hydrolase from Fusarium oxysporum LCH1 and Fusarium solani f. sp. pisi. Biotechnology Journal, 2:361-364.

Qi Q, Mokhtar MN, Zimmermann W. 2007. Effect of ethanol on the synthesis of large-ring cyclodextrins by cyclodextrin glucanotransferases. Journal of Inclusion Phenomena and Macrocyclic Chemistry, 57:95-99.

Kaulpiboon J, Pongsawasdi P, Zimmermann W. 2007. Molecular imprinting of cyclodextrin-glycosyltransferases from Paenibacillus sp. A11 and Bacillus macerans with g-cyclodextrin. FEBS Journal, 274:1001–1010.


Chaiyaso T, H-Kittikun A, Zimmermann W. 2006. Biocatalytic acylation of carbohydrates with fatty acids from palm fatty acid distillates. Journal of Industrial Microbiology & Biotechnology, 33:338-342.

Alisch-Mark M, Herrmann A, Zimmermann W. 2006. Increase of the hydrophilicity of polyethylene fibres by hydrolases from Thermomonospora fusca and Fusarium solani f. sp. pisi. Biotechnology Letters, 28: 681-685.


Andreaus J, Barcellos IO, Blosfeld AM, Josefowicz M, Fischer CH, Wilhelm S, Budag N, Alisch M, Zimmermann W. 2005. Modificação quimica e biocatalítica de poliéster. Química Têxtil 79:55-66.

Qi Q, Zimmermann W. 2005. Cyclodextrin glucanotransferase: from gene to application. Applied Microbiology and Biotechnology 66:475-485.

2004 und früher

Alisch M, Feuerhack A, Müller, H, Mensak, B, Andreaus J, Zimmermann W. 2004. Biocatalytic modification of polyethylene terephthalate fibres by esterases from actinomycete isolates. Biocatalysis and Biotransformation 22:347-351.

Qi Q,  She X, Endo T, Zimmermann W. 2004. Effect of the reaction temperature on the transglycosylation reactions catalyzed by the cyclodextrin glucanotransferase from Bacillus macerans for the synthesis of large-ring cyclodextrins. Tetrahedron 60:799-806.

Zheng M, Endo T, Zimmermann W. 2002. Synthesis of large-ring cyclodextrins by cyclodextrin glucanotransferases from bacterial isolates. Journal of Inclusion Phenomena and Macrocyclic Chemistry 44:387-390.

Endo T, Zheng M, Zimmermann W. 2002. Production and analysis of large-ring cyclodextrins. Aust J Chem 55:39-48.

Degn P, Zimmermann W. 2001. Optimisation of carbohydrate fatty acid ester synthesis in organic media by a lipase from Candida antarctica. Biotechnology and Bioengineering 74:483-491.

Duedahl-Olesen L, Kragh KM, Zimmermann W. 2000. Purification and characterisation of a malto-oligosaccharide-forming amylase active at high pH from Bacillus clausii BT-21.  Carbohydrate Research 329:97-107.

Degn P, Larsen KL, Duus JO, Petersen BO, Zimmermann W. 2000. Two-step enzymatic synthesis of malto-oligosaccharide  esters. Carbohydrate Research 329:57-63.

Duedahl-Olesen L, Larsen KL, Zimmermann W. 2000. Rapid detection of malto-oligosaccharide-forming bacterial amylases by high performance anion-exchange chromatography. Letters in Applied Microbiology 30:312-316.

Reuter S, Nygaard AR, Zimmermann W. 1999. b-Galactooligosaccharide synthesis with b-galactosidases from Sulfolobus solfataricus, Aspergillus oryzae, and Escherichia coli. Enzyme and Microbial Technology 25:509-516.

Degn P, Pedersen LH, Duus JØ, Zimmermann W. 1999. Lipase-catalyzed synthesis of glucose fatty acid esters in tert-butanol. Biotechnology Letters, 21:275-280.

Larsen KL, Zimmermann W. 1999. Analysis and characterization of cyclodextrins and their inclusion complexes by affinity capillary electrophoresis. Journal of Chromatography A 836:3-14.

Duedahl-Olesen L, Zimmermann W, Delcour JA. 1999. Effects of low molecular weight carbohydrates on farinograph characteristics and staling endotherms of yeastless wheat flour doughs. Cereal Chemistry 76:227-230.

Mørkbak AL, Degn P, Zimmermann W. 1999. Deinking of soy bean oil based ink printed paper with lipases and a neutral surfactant. Journal of Biotechnology 67:229-236.

Bassompierre M, Børresen T, Sandfield P, Rønsholdt B, Zimmermann W, McLean E. 1997. An evaluation of open and closed systems for in vitro protein digestion of fish meal. Aquaculture Nutrition 3:153-159.

Pedersen LH, Christensen HJS, Mathiesen F, Larsen KL and Zimmermann W. 1997. Interference of cyclodextrins with amylolytic activity assays of cyclodextrin glycosyltransferases. Starch 49:250-253.

Larsen KL, Mathiesen F, Zimmermann W. 1997. Separation and analysis of cyclodextrins by capillary zone electrophoresis. Carbohydrate Research 298:59-63.

Kildemark N, Larsen KL, Zimmermann W. 1996. Complex formation of unsaturated cyclodextrin solutions with various polymers. Journal of Inclusion Phenomena and Molecular Recognition in Chemistry 25:89-92.

Hilge M, Gloor S, Winterhalter, K, Zimmermann W, Piontek K. 1996. Crystallization and preliminary crystallographic analysis of two beta-mannanase isoforms from Thermomonospora fusca KW3. Acta Crystallographica  D52:1224-1225.

Brühlmann F, Kim K, Zimmermann W, Fiechter A. 1994.  Pectinolytic enzymes from actinomycetes for the degumming of ramie bast fibres. Applied and Environmental Microbiology 60:2107-2112.

Zhou W, Winter B, Zimmermann W. 1993. Dechlorination of high-molecular-mass compounds in spent sulfite bleach effluents by free and immobilized cells of streptomycetes. Applied Microbiology and Biotechnology 39: 418-423.

Zhou W, Zimmermann W. 1993. Decolorization of industrial effluents containing reactive dyes by actinomycetes. FEMS Microbiology Letters 107:157-162.

Studer M, Flück K, Zimmermann W. 1992. Production of endo- and exochitinases by Aphanocladium album grown on crystalline and colloidal chitin. FEMS Microbiology  Letters 99:213-216.

Röthlisberger P, Fiechter A, Zimmermann W. 1992.  Production of thermostable xylanases in batch and continuous cultures by Thermomonospora fusca KW3. Applied Microbiology and Biotechnology 37:416-419.

Tajana E, Fiechter A, Zimmermann W. 1992. Purification and characterization of two alpha-L-arabinofuranosidases from Streptomyces diastaticus. Applied and Environmental Microbiology 58:1447-1450.

Mliki A, Zimmermann W. 1992. Purification and characterization of an intracellular peroxidase from Streptomyces cyaneus. Applied and Environmental Microbiology 58:916-919.

Zimmermann W. 1991. Enzymic solubilisation of [14C]-lignin-labeled lignocellulose. Mitteilungen BFA für Forst- und Holzwirtschaft Hamburg 168:167-170.

Constam D, Muheim A, Zimmermann W., Fiechter A. 1991. Purification and characterization of an intracellular NADH:quinone oxidoreductase from P. chrysosporium. Journal of  General  Microbiology 137:2209-2214.

Winter B, Fiechter A, Zimmermann W. 1991. Degradation of organochlorine compounds in spent sulfite bleach plant effluents by actinomycetes. Applied and Environmental Microbiology 57:2858-2863.

Zimmermann W. 1990. Degradation of lignin by bacteria. Journal of Biotechnology 13:119-130.

Zimmermann W. 1989. The lignin biopolymer: products and potential applications of lignin biodegrading systems. Chimia 43:396-403.

Zimmermann W, Broda P. 1989. Utilization of lignocellulose from barley straw by actinomycetes. Applied Microbiology and Biotechnology 30:103-109.

Zimmermann W, Umezawa T, Broda P, Higuchi T. 1988. Degradation of a non-phenolic arylglycerol beta-aryl ether by Streptomyces cyaneus. FEBS Letters 239:5-7.

Zimmermann W, Winter B, Broda P. 1988. Xylanolytic enzyme activities produced by mesophilic and thermophilic actinomycetes grown on graminaceous xylan and lignocellulose. FEMS Microbiology Letters, 55:181-186.

Mason JC, Richards M, Zimmermann W, Broda P. 1988. Identification of extracellular proteins from actinomycetes responsible for the solubilisation of lignocellulose. Applied Microbiology and Biotechnology, 28:276-280.

Zimmermann W, Nimz H, Seemüller E. 1985. 1H and 13C NMR spectroscopic study of extracts from corks of Rubus idaeus, Solanum tuberosum, and Quercus suber. Holzforschung 39:45-49.

Zimmermann W, Seemüller E. 1984. Degradation of raspberry suberin by Fusarium solani f. sp. pisi and Armillaria mellea. Phytopathologische Zeitschrift 110:192-199.

Fernando G, Zimmermann W, Kolattukudy PE. 1984. Suberin-grown Fusarium solani f. sp. pisi generates a cutinase-like esterase which depolymerizes the aliphatic components of suberin. Physiological Plant Pathology 24:143- 155.

letzte Änderung: 19.05.2017


Prof. Dr. Wolfgang Zimmermann
Institut für Biochemie
Johannisallee 21-23
D-04103 Leipzig

Telefon: +49 341 97-36781
Telefax: +49 341 97-36798

Susanne Vogt

Telefon: +49 341 97-36780
Telefax: +49 341 97-36798